Identification and characterization of three novel small interference RNAs that effectively down-regulate the isolated nucleocapsid gene expression of SARS coronavirus.
Identifieur interne : 002105 ( Main/Exploration ); précédent : 002104; suivant : 002106Identification and characterization of three novel small interference RNAs that effectively down-regulate the isolated nucleocapsid gene expression of SARS coronavirus.
Auteurs : Ying-Li Cao [République populaire de Chine] ; Ying Wang ; Rong Guo ; Fan Yang ; Yun Zhang ; Shu-Hui Wang ; Li LiuSource :
- Molecules (Basel, Switzerland) [ 1420-3049 ] ; 2011.
Descripteurs français
- KwdFr :
- Alignement de séquences, Animaux, Cellules HEK293, Cellules Vero, Données de séquences moléculaires, Expression des gènes, Humains, Lignée cellulaire, Petit ARN interférent (génétique), Petit ARN interférent (métabolisme), Protéines nucléocapside (génétique), Protéines nucléocapside (métabolisme), Régulation négative, Séquence nucléotidique, Virus du SRAS (génétique), Virus du SRAS (métabolisme).
- MESH :
- génétique : Petit ARN interférent, Protéines nucléocapside, Virus du SRAS.
- métabolisme : Petit ARN interférent, Protéines nucléocapside, Virus du SRAS.
- Alignement de séquences, Animaux, Cellules HEK293, Cellules Vero, Données de séquences moléculaires, Expression des gènes, Humains, Lignée cellulaire, Régulation négative, Séquence nucléotidique.
English descriptors
- KwdEn :
- Animals, Base Sequence, Cell Line, Chlorocebus aethiops, Down-Regulation, Gene Expression, HEK293 Cells, Humans, Molecular Sequence Data, Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (metabolism), RNA, Small Interfering (genetics), RNA, Small Interfering (metabolism), SARS Virus (genetics), SARS Virus (metabolism), Sequence Alignment, Vero Cells.
- MESH :
- chemical , genetics : Nucleocapsid Proteins, RNA, Small Interfering.
- chemical , metabolism : Nucleocapsid Proteins, RNA, Small Interfering.
- genetics : SARS Virus.
- metabolism : SARS Virus.
- Animals, Base Sequence, Cell Line, Chlorocebus aethiops, Down-Regulation, Gene Expression, HEK293 Cells, Humans, Molecular Sequence Data, Sequence Alignment, Vero Cells.
Abstract
Nucleocapsid (N) protein of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is a major pathological determinant in the host that may cause host cell apoptosis, upregulate the proinflammatory cytokine production, and block innate immune responses. Therefore, N gene has long been thought an ideal target for the design of small interference RNA (siRNA). siRNA is a class of small non-coding RNAs with a size of 21-25nt that functions post-transcriptionally to block targeted gene expression. In this study, we analyzed the N gene coding sequences derived from 16 different isolates, and found that nucleotide deletions and substitutions are mainly located at the first 440nt sequence. Combining previous reports and the above sequence information, we create three novel siRNAs that specifically target the conserved and unexploited regions in the N gene. We show that these siRNAs could effectively and specifically block the isolated N gene expression in mammal cells. Furthermore, we provide evidence to show that N gene can effectively up-regulate M gene mediated interferon b (IFNb) production, while blocking N gene expression by specific siRNA significantly reduces IFNb gene expression. Our data indicate that the inhibitory effect of siRNA on the isolated N gene expression might be influenced by the sequence context around the targeted sites.
DOI: 10.3390/molecules16021544
PubMed: 21317844
Affiliations:
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Le document en format XML
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sort="Wang, Ying" uniqKey="Wang Y" first="Ying" last="Wang">Ying Wang</name></author><author><name sortKey="Guo, Rong" sort="Guo, Rong" uniqKey="Guo R" first="Rong" last="Guo">Rong Guo</name></author><author><name sortKey="Yang, Fan" sort="Yang, Fan" uniqKey="Yang F" first="Fan" last="Yang">Fan Yang</name></author><author><name sortKey="Zhang, Yun" sort="Zhang, Yun" uniqKey="Zhang Y" first="Yun" last="Zhang">Yun Zhang</name></author><author><name sortKey="Wang, Shu Hui" sort="Wang, Shu Hui" uniqKey="Wang S" first="Shu-Hui" last="Wang">Shu-Hui Wang</name></author><author><name sortKey="Liu, Li" sort="Liu, Li" uniqKey="Liu L" first="Li" last="Liu">Li Liu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21317844</idno><idno type="pmid">21317844</idno><idno type="doi">10.3390/molecules16021544</idno><idno type="wicri:Area/PubMed/Corpus">001552</idno><idno type="wicri:explorRef" wicri:stream="PubMed" 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sort="Zhang, Yun" uniqKey="Zhang Y" first="Yun" last="Zhang">Yun Zhang</name></author><author><name sortKey="Wang, Shu Hui" sort="Wang, Shu Hui" uniqKey="Wang S" first="Shu-Hui" last="Wang">Shu-Hui Wang</name></author><author><name sortKey="Liu, Li" sort="Liu, Li" uniqKey="Liu L" first="Li" last="Liu">Li Liu</name></author></analytic><series><title level="j">Molecules (Basel, Switzerland)</title><idno type="eISSN">1420-3049</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Down-Regulation</term><term>Gene Expression</term><term>HEK293 Cells</term><term>Humans</term><term>Molecular Sequence Data</term><term>Nucleocapsid Proteins (genetics)</term><term>Nucleocapsid Proteins (metabolism)</term><term>RNA, Small Interfering (genetics)</term><term>RNA, Small 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scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term><term>Animaux</term><term>Cellules HEK293</term><term>Cellules Vero</term><term>Données de séquences moléculaires</term><term>Expression des gènes</term><term>Humains</term><term>Lignée cellulaire</term><term>Régulation négative</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Nucleocapsid (N) protein of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is a major pathological determinant in the host that may cause host cell apoptosis, upregulate the proinflammatory cytokine production, and block innate immune responses. Therefore, N gene has long been thought an ideal target for the design of small interference RNA (siRNA). siRNA is a class of small non-coding RNAs with a size of 21-25nt that functions post-transcriptionally to block targeted gene expression. In this study, we analyzed the N gene coding sequences derived from 16 different isolates, and found that nucleotide deletions and substitutions are mainly located at the first 440nt sequence. Combining previous reports and the above sequence information, we create three novel siRNAs that specifically target the conserved and unexploited regions in the N gene. We show that these siRNAs could effectively and specifically block the isolated N gene expression in mammal cells. Furthermore, we provide evidence to show that N gene can effectively up-regulate M gene mediated interferon b (IFNb) production, while blocking N gene expression by specific siRNA significantly reduces IFNb gene expression. Our data indicate that the inhibitory effect of siRNA on the isolated N gene expression might be influenced by the sequence context around the targeted sites.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Guo, Rong" sort="Guo, Rong" uniqKey="Guo R" first="Rong" last="Guo">Rong Guo</name><name sortKey="Liu, Li" sort="Liu, Li" uniqKey="Liu L" first="Li" last="Liu">Li Liu</name><name sortKey="Wang, Shu Hui" sort="Wang, Shu Hui" uniqKey="Wang S" first="Shu-Hui" last="Wang">Shu-Hui Wang</name><name sortKey="Wang, Ying" sort="Wang, Ying" uniqKey="Wang Y" first="Ying" last="Wang">Ying Wang</name><name sortKey="Yang, Fan" sort="Yang, Fan" uniqKey="Yang F" first="Fan" last="Yang">Fan Yang</name><name sortKey="Zhang, Yun" sort="Zhang, Yun" uniqKey="Zhang Y" first="Yun" last="Zhang">Yun Zhang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Cao, Ying Li" sort="Cao, Ying Li" uniqKey="Cao Y" first="Ying-Li" last="Cao">Ying-Li Cao</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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